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Wednesday, 08 January 2014 20:59

Isolated organ/tissue test – organ bath

The goal of a drug discovery effort is to generate novel, highly selective molecules that either inhibit or activate processes in the cell to ultimately produce therapeutic effects in humans. 

Wednesday, 08 January 2014 14:12

THERMOMETER - DIGITAL (Panlab)

Panlab proposes two multi-channel digital thermometer models suitable for the measurement of animal body temperature using rectal probes. Both systems are designed for working with up to 12 resistive-temperature probes simultaneously.

Temperature is measured by using Yellow Springs (YSI) thermistor and the registered current value shown in the digital display of the front panel with a resolution of 0.1 degrees (sensitivity range: 32-45°C). There is no difference between channels or temperature probes, freely interchangeable. Standard YSI temperature probes should be plugged in the jack connectors of the rear panel, while the temperature value is read from the front panel display.

TMP812 model allows the reading of the current temperature of one channel at once. The selection is done by switching the desired channel in the 12-positions front panel rotary commuter.

TMP812RS model offers a 20x4 LCD display for showing the current temperature of all the probes at the same time. A built-in RS 232/USB communication port, allows sending (by pressing a front panel “SEND” button) the temperature values from all the probes to an external device (matrix printer with serial port or PC computer) through the SEDACOM software.

The new SEDACOM 2.0 version provides an easy and convenient way to visualize and export the data on a computer for further analysis.

 


Key features

  • Precalibrated and interchangeable probes
  • Options for Data transfer to a computer or printer

 


Specifications

Resolution

0,1 degrees Celsius

Capacity

Up to12 temperature probes simultaneously

Temperature probes

Standard YSI probes

Communications

RS232 (USB)

  


How to order 

CONTROL UNIT

TMP812RS

76-0065

12 Channels Digital Thermometer with RS232 port comms. Probes and SeDaCom software should be ordered separately.

 

TEMPERATURE PROBES

YS401

76-0066

Temperature probe for rabbits (3.9 mm)

YS402

76-0067

Temperature probe for rats & mice (3 mm)

YS451

76-0068

Temperature probe for mice (1.3 mm)

YS409

76-0069

Surface temperature probe (disc dia. 9.5 mm)

 

DATA TRANSFER & SOFTWARE

SEDACOM V2.0

76-0406

SeDaCom V2.0 software for data transfer to a computer

CONRS232USB

76-0608

SeDaCom accessory - RS232/USB adapter

  


 Body temperature

See more information about body temperature evaluation in rodents in next link

 


 

Tuesday, 07 January 2014 20:02

DIGITAL STIMULATORS (Panlab)

Panlab provides a complete set of precise, compact and easy-to-use single-, double- or four-channels digital stimulators for general pharmacology and physiology such as organ/tissue bath studies. 

With a wide range of stimulation parameters, these stimulators offer an excellent and low priced alternative when single pulse and train stimulation is required.

 


Key features

  • Pulse and Train formation entirely controlled by Microprocessor
  • Allows stimulation of more than one isolated tissue preparation
  • Stimulation by Voltage and Current
  • Visual short-circuit security warning
  • Square-wave pulse
  • Built-in isolated output circuit (not related to ground)
  • Selected-parameters violation warning
  • Monopulse, Continuous and Train stimulations
  • TTL signal allowing for the external control of
  • DC Output
  • 125/220-50/60Hz power supply

 


Specifications

 

BASIC STIMULATOR LE12006

Number of Channels

1 channel

Pulse Frequency

0.01 - 99.99 Hz (0.01 Hz step)

Pulse Duration

0.1 - 9.9 ms (0.1 ms step)

OUTPUT Voltage

0 - 50 V (1 V resolution)

OUTPUT Max. Power

15 W (250 mA)

Accuracy

≤ 4% for timings and output

Power Supply

125/220V, 50/60Hz

 

PULSE GENERATOR

 

PULSE Frequency

1 Hz - 10 KHz (1 Hz step)

PULSE Duration

1 - 9999 μs (1 μs step); 1 - 9999 ms (1 ms step)

TRAIN Interval

0.1 - 999.9 s (1 s step)

TRAIN Duration

1 - 99.99 s (0.01 s step)

DIGITAL OUTPUT

5 V (TTL)

Accuracy

≤ 2% for timings and output

 

POWER UNIT

 

OUTPUT Voltage

0 - 100 V (1 V resolution); constant voltage (automated short circuit security)

OUTPUT Current

0 - 500 mA (5 mA resolution); constant current (automated short circuit security). Except for LE 12106: 0 - 100 mA (1 mA resolution); constant current (automated short circuit security)

OUTPUT Max

Power 50 W per channel (500 mA)

 

  


How to order 

 

COMPACT SYSTEMS

Dimensions (W x D x H)

LE12006

76-0061

1-Channel Basic Stimulator for teaching purpose

29 x 27 x 7 cm

LE12106

76-0055

1-Channel Digital Stimulator including 1 Pulse generator and 1 Power unit

34 x 31 x 15 cm

LE12206

76-0057

2-Channels Stimulator including 2 Pulse generators and 2 Power units

45 x 35 x 19 cm

LE12406

 76-0587

4-Channels Stimulator including 1 Pulse generator and 4 Power units

45 x 35 x 19 cm

 

MODULAR SYSTEMS

 

LE12406OM

76-0058

Power Unit module (LE12406TC needed)

7 x 12 x 17 cm

LE12406TC

76-0059

Pulse Generator & Mainframe module for up to 4 LE12406OM units

45 x 35 x 19 cm

LE12404

76-0060

Mainframe module for up to 4 LE12406OM units (External Pulse generator needed)

45 x 35 x 19 cm

LE12806

 

8-Channels Stimulator package including 1 LE12406TC, 1 LE12404 and 8 LE12406OM

2 x (45 x 35 x 19 cm)

 

OTHER ACCESSORIES

TRS081

76-0062

Nerve Electrode

TRS082

76-0063

Bipolar Electrode

 

  


Related products

  • Organ bath for teaching (P) - link
  • Organ bath for basic research (P) - link
  • Organ bath for advanced research (HS)

 


 

Tuesday, 07 January 2014 17:53

ISO510A AMPLIFIER (Panlab)

The PANLAB Coupler Module ISO510A is an Amplifier for bridge-system designed to work with a wide range of different transducers for evaluating pressure, strength or respiration in various research areas. As an example, the ISO510 model is currently associated with the isometric or isotonic transducers for the study of in vitro tissues preparations using Organ Baths.

The PANLAB ISO510A Amplifier can work in both AC and DC and amplifies signal up to 2.000 times. The Amplifier contains a filter allowing the suppression of the noise produced by electrical or mechanical artefacts associated with the experiment running (for instance, ventilation and heating pump systems in Organ Baths) and then eliminates vibrations that may affect the signal received from the biological preparations. A Transducer Balance option allows a fine adjustment of the signal baseline (“zero”) when the transducer is not excited by the variable to be measured. The ISO510A amplifier also allows the external monitoring of its output signal, for instance to an oscilloscope…

The ISO510A Amplifier is available in two versions: individual Amplifiers accompanied with the corresponding external Power Supply Box or individual Amplifiers integrated into the Power Supply Box.

 


Key features

  • High Gain Differential AC/DC Amplifier
  • Includes a filter allowing the suppression of background noise
  • A Transducer Balance allows a fine adjustment of the signal baseline
  • Allows external monitoring of its output signal

 


Specifications

Gain

0-2000

Input Impedance

1.5 kOhms

Output Impedance

< 100 Ohms

Feed Power

18 VDC

Output Voltage

10 V

Transducer Power Supply

10 VDC

Band Frequency

0-5 KHz

Filters

>3 Hz or >20 Hz

Filter AC

0.16 Hz

Connector

5 pin Female Audio Connector

Certification

CEE Compliance

 

 


How to order 

ISO510A

76-0627

Needs a Power Supply Box

BR4740A

76-0586

Power Supply Box for up to 4 Amplifiers ISO510A

 


Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

 


Related products

  • Transducers (P) – link
  • PROTOWIN software (P) - link
  • Organ bath for teaching (P) - link
  • Organ bath for basic research (P) - link
  • Organ bath for advanced research (HS)

 


 

Tuesday, 07 January 2014 14:13

TRANSDUCERS (Panlab)

Panlab provides complete hardware solutions and associated software for isolated tissues studies from simple, manual systems to automated, computer-controlled systems. 

The mechanical activity of the tissues (muscles) is formed by a displacement (elongation) and a force (contraction). The ratio between elongation and force depends on the properties of the tissue under study: some tissues exhibit a mechanical response with a very large displacement and a reduced force; other tissues produce short but very intense contractions (in terms of strength). Depending on this ratio, the transducer used for performing an organ bath experiment will be isometric or isotonic.

- Isometric transducers

The Panlab Force/Isometric Transducers measures the force of contraction of isolated tissues under conditions of constant force. 

Isometric force transducers are very sensitive to the force applied under conditions contraction with very short amplitude (low displacement). As an example, the Transducers of weight of the electronic scales acts in an isometric way. Generally the isometric technology is also used to measure frequencies; for this reason, the displacement of the sensor of the transducer need to be as slight as possible. Panlab has transducers with a minimum displacement of 0.1 mm for a force application until 25 grams.

Isometric Transducers are classically used in Organ Bath experiments with muscles with short/rapid contractions such as tracheal muscle, atrium, papillary muscle, aortic rings, veins or other vessel rings.

The Transducers are equipped with a special system allowing an easy and rapid fixation of the thread that tightens upper side of the tissue in an organ bath. Panlab provides transducer for various load range, from teaching to standard research systems.

- Isotonic transducers

The Panlab Isotonic Transducer measures displacements with pre-adjusted loads (position transducer).

Isotonic transducers are classically used for measuring the contraction amplitude of isolated muscles in Organ Bath studies, i.e. the changes in tissue length observed under a constant load.

To achieve this capacity, an isotonic transducer must possess a sensor axis which rotates at least 25 degrees. This type of register was the one which was used initially, at the time of the kymographs in such a way that the transducer was configured by a lever, balancing on a pivot.

These transducers are preferably used with smooth muscle preparations characterized by relatively slow contractions (ileum, vas deferens, lung strips, esophagus, urinary bladder, etc.).

Download here the Transducer leaflet – link

 


Which transducer? The Panlab advice.

Although in principle and from the physiological point of view, the most appropriate for smooth musculature is the isotonic and for heart and striate the isometric, in purity all animal muscle contracts partly isotonically and partly isometrically. Nowadays all the tasks are done with isometric as the response is much more repetitive, the basal line is much more constant, and the relaxation much more rapid. Isotonic transducers are only used in few specific cases

Recommended bibliography:

Kitchen I. Textbook of in vitro practical pharmacology. Blackwell Scientific Publications. Oxford, 1984

 


Panlab transducer specifications

 

Type

Isometric (Force)

Isotonic

 

Model

TRI201

TRI210

TRO015

 

Feature

High sensitivity

Teaching

Displacement

 

Range

5 mg to 25 g

10 mg to 25 g

5 mg to 5 g

 

Resolution

2 mg

5 mg

5 mg

 

Displacement

± 0.45 mm

± 0.5 mm

± 15 º (48 mm)

 

Sensitivity

32 μV/V/g

25 μV/V/g

24 μV/V/º

 

Power Supply

5-10 V dc/ac

5-10 V ac/dc

5-10 V dc

 

ROD Length (6mm dia.)

165 mm

165 mm

150 mm

 

Counterweight graduation

 

 

5 div of 1 g with subdiv of 250 mg

 

 

 


How to order 

 

5 pin female audio connector

6 pin male binder connector

8 pin male connector

 

Transducer connector for Panlab ISO510A amplifier and ADinstrument Bridge pod.

Transducer connector for Hugo Sachs amplifiers.

Transducer connector for ADinstruments amplifiers.

 

TRI201A (76-0575)

TRI201HS (76-0418)

TRI201AD (76-0417)

 

TRI210A (76-0098)

TRI210HS (76-0579)

TRI210AD (76-0581)

 

TRO015A (76-0535)

TRO015HS (76-0424)

TRO015AD (76-0423)

 

Five pin male connectors for Panlab previous ISO510 amplifier are obsolete but can be provided under specific request.   

 


Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

 


Related products

  • Amplifiers (P) – link
  • PROTOWIN software (P) - link
  • Organ bath for teaching (P) - link
  • Organ bath for basic research (P) - link
  • Organ bath for advanced research (HS)

 


 

Tuesday, 07 January 2014 09:56

PROTOWIN Software (Panlab)

Panlab provides complete hardware solutions and associated software for isolated tissues studies from simple, manual systems to automated, computer-controlled systems. 

PROTOWIN is a complete software for monitoring isolated tissue experiments. It represents a very powerful tool saving the user time and effort in the awkward calculations involved in this research area. Its interactive design makes it very easy to use and its extensive features make it an essential tool for teaching and research.

Basically, PROTOWIN software allows the acquisition and analysis of signals generated by the contraction of isolated organ preparations maintained into an Organ Bath and challenged by drugs exposure or electrical stimulation. 

The software can analyze and store signals form up to 4 channels (i.e. from up to 4 tissue preparations) simultaneously or in an independent manner. The analogical signal charts can be viewed on the screen one by one or in sets of four units at a time. Independent adjustment of full scale range, time axis and offset are available for each channel, as well as independent base–maximum plot for each point.

The system facilitates the extraction of data into a spreadsheet for further analysis. Regular data Back-up is automatically performed as a safety measure in case of accidental computer shutdown.

 


Key features

  • Simultaneous or independent start-stop for each channel (tissue)
  • Data given separately for up to 16 channels (tissues preparations)
  • Simple, intuitive and guided channel calibration and balancing process

 


How to order

 

PROTOWIN V1.1

76-0038

Software for Organ Bath. Up to 4 channels. Dose/Response.

 


Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

 


Related products

  • Transducers (P) – link
  • Amplifiers (P) – link
  • Organ bath for basic research (P) – link

 


 

Panlab provides complete hardware solutions and associated software for isolated tissues studies from simple, manual systems to automated, computer-controlled systems. 

The Panlab Compact Organ Baths have been designed and developed to satisfy the requirements of investigators by offering advanced characteristics that render them suitable for the in-vitro study of tissue behavior. Due to their compact design minimum bench space is required.

The modular design allows up to eight individually mounted tissues to be studied simultaneously and independently per system. Several models are available, from one up to eight chambers. All of them are supplied with an external heating control unit, transducer' stands & micropositioners and tissue holders. The main Perspex reservoir houses the heater system, the chambers and coils, all submerged in thermostatically controlled water. Each vessel is filled and emptied by means of a three-way tap (Manual Series) or by electrically-operated valves (Automatic Series) which, besides the possibility of manual activation by means of frontal pushbuttons, can also be remotely controlled by a Timer or Programmer (Software).

All organ baths include tissue holders, transducer holders, micropositioners. The tissue chambers are sold separately.

Panlab can too supply suitable Isometric or Isotonic Force Transducers.

Standard solution for teaching uses the LE120-1 or LE120-2 flat recorders for recording the signals on paper. Optionally, the PANLAB Modular Organ Baths can be associated with transducers, amplifiers and data output software from several brands. 

Cost-saving and straight-to-the-point solution is available as a combination with the Panlab transducers, amplifiers and PROTOWIN software. 

The Panlab organ baths are also the main piece of the ADInstruments organ baths working with the Powerlab and LABChart software systems and proving advanced data report options.

Download here the Panlab Organ bath leaflet – link

 


Key features

  • Compact design thus reducing space requirements to the minimum
  • 5, 10, 25 and 50 ml chambers available
  • Complete accessibility to all components to facilitate maintenance
  • Independent nutrient liquid inputs allowing using different physiological solutions in one same chamber
  • All the input-output connections are accessible on the rear panel of the device
  • Additional gas outlet (bubbling) to supply oxygen to the preparation into the Petri Capsules
  • Two modes for solution replacement: overflow / emptying and filling
  • Turbulence-free water circulation
  • Use of safety sensors for both water level and water temperature (cut-off at 50 ºC)
  • Avoid of thermal inertia using an electronic heating resistance operation control system with incremental gradients
  • Available accessories for using continuous perfusion
  • Ensures optimal heat stability
  • Use of totally inert materials, PTFE, Delrin, Silicone and other materials that are not degraded by acids or salts

 


How to order

 

 

COMPACT ORGAN BATHS (Chambers not Included)

Chambers

Dimensions (cm)

Tank (liters)

Manual

Automatic

2

Base: 28 x 20; Height: 27 + 22

5 L

LE01002

76-0030

LE01026

76-0031

4

Base: 44 x 20; Height: 27 + 22

8 L

LE01004

76-0032

LE01046

76-0033

6

Base: 60 x 20; Height: 27 + 22

11 L

LE01006

76-0034

LE01066

76-0035

8

Base: 76 x 20; Height: 27 + 22

14 L

LE01008

76-0036

LE01086

76-0037

 

CHAMBERS

LE0105

76-0044

5 mL

LE0110

76-0045

10 mL

LE0125

76-0046

25 mL

LE0150

76-0047

50 mL

 

 

ELECTRODES & STIMULATORES

LE01030A

76-0048

Stainless steel electrode

LE01030B

76-0049

Stainless steel electrode. Two rings, 20 mm. apart

LE01030C

76-0050

Stainless steel electrode. Two rings, 15 mm apart

LE01030D

76-0051

Stainless steel electrode. Surface contact

LE01035A

76-0052

Platinum electrode. Single ring

LE01035B

76-0053

Platinum electrode. Two rings, 20 mm apart

LE01035C

76-0054

Platinum electrode. Two rings, 15 mm apart

LE01035D

76-0393

Platinum electrode. Surface contact

STIMULATORS

 

See available Panlab options in next link

 

TRANSDUCERS (see Related product)

 

AMPLIFIERS (see Related products)

  

DATA OUTPOUT

PROTOWIN V1.1

76-0038

Software for Organ Bath. Up to 4 channels. Dose/Response.

  


Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

 


Related products

  • Transducers (P) –link
  • Amplifiers (P) – link
  • PROTOWIN Software (P) - link
  • Organ bath for Teaching (P) - link
  • Organ bath for advanced research (H)

 


 

Wednesday, 09 April 2014 00:00

METABOLISM Software

METABOLISM software is the last element of the chain of components of the Panlab OXYLET,FOOD and DRINK and PHYSIOCAGE systems. METABOLISM allows the extraction of the data obtained from these Panlab devices as well as the calculation of important parameters for physiological studies. A combined evaluation of the respiration metabolism, food/drink intake, spontaneous activity and rearing is then rendered possible by the use of this very simple and easy-to-use software.

METABOLISM consists in different modules, activated upon customer request:

  • METAOXY -> for O2/CO2 metabolism studies (Including Treadmill based studies).
  • METAINT -> for Intake studies
  • METAACT -> for activity studies (Including Rearing)

The program gets data in digital form from the Panlab devices. METABOLISM can also import and convert analogical data from the *.txt files generated by Data Acquisition Systems (PowerLab® recommended). 

Gathered data can be processed and re-processed using different time intervals of calculation. The program displays a Data Table which can be saved in Excel format for further analyses.

 

Tuesday, 01 April 2014 00:00

COMPULSE Software

Compulse represents the software component of the Panlab PheCOMP system designed for the assessment of compulsive behavior in rodents.

This software monitors up to 12 PheCOMP cages for data acquisition, storage and analysis. Up to 4 external units for food or drink by cage can be set (user-defined combination and name) ensuring an optimal flexibility for performing a wide variety of tests related to Food & Drink Intake studies.

The evolution of food and drink intake consumption, beams break activity, rearing and current animal position are displayed in real-time during the data acquisition process. The data are stored in two data files: MTB file opened from the Compulse software for Intake & Meal Pattern Analysis and TAC file opened from the Actitrack software for Global Activity, Tracking & Rearing Analysis.

A powerful analysis module provides tables with the calculation of total and accumulated intake for each dispenser in each cage as well as all the standard data for meal pattern analysis. The analysis window and subinterval are defined by the user. Data can also be displayed graphically. All the tables and graphs are easily exportable in Excel o BMP formats, respectively.

 

 

Tuesday, 08 April 2014 00:00

PLACE PREFERENCE BOX – Spatial

The Panlab spatial place preference box is an experimental chamber developed with the aim to optimise place preference and aversion studies in small laboratory animals, especially mice. The design of the box is fruit of a close collaboration with eminent Professor Dr. Rafael Maldonado and Dr. Olga Valverde from the Laboratory of Neuropharmacology in Barcelona (Spain). 

The apparatus consists in a box with two equally sized compartments interconnected by a rectangular corridor. Originally, the compartments are differentiated by the motifs painted on the walls (dots or stripes) and the colour (different shade of grey tones, light or dark) and texture (smooth or rough) of the floor. The innovation brought by the Panlab box is the possibility to combine a new additional spatial dimension to allow the animal differentiate the different compartments in a more discriminative manner. Transparent walls are also used to minimize the time the animal spent in the corridor. 

The introduction of these new discriminative elements allows:

  • optimising the results obtained in the place preference and aversion paradigms (low variability in the response, reduced number of animals per group...)
  • organizing the discriminative elements in a wide variety of configurations for studies evaluating spatial or contextual memory
  • using the elements as discriminative cues associated with drug exposure in diverse other experimental designs.

The Panlab spatial place preference box can be associated with the weight transducer technology (and PPC win software) or video-tracking system (SMART software) for detection and analysis of animal position throughout the test.

 

 
 
 
 
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