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OXYLETPRO - PHYSIOCAGE

Para el estudio del metabolismo respiratorio, la ingesta y la actividad en roedores

Descripción
Puntos clave
Especs.
Como pedirlo
Publicaciones

Descripción

Innovative Metabolic Monitoring

OxyletPro is a modular system integrating:

  • Respiratory metabolism (VO2 consumption/VCO2 production)
  • Food and drink intake
  • Activity/rearing measurements

OxyletPro, an optimized system for studies in laboratory research models, utilizes indirect calorimetry to evaluate respiratory metabolism.

Food and drink intake and activity are evaluated using Panlab’s weight transducer technology. This highly stable technology permits the continuous assessment of consumption and spontaneous activity with superior accuracy. For a more comprehensive evaluation of activity, an Infrared (IR) frame can be added to monitor rearing behavior.

OxyletPro’s unique modular design allows for simple expandability. Start with the configuration that meets your requirements today, and expands as needs change and grow.

Special configurations are available for calorimetry studies with neonatal rat pups as well as exercise physiology studies with our single lane, airtight treadmills.

OxyletPro can be used in the following applications:

Obesity, diabetes, metabolic disorders, nutrition studies, chronobiology/circadian rhythm studies, drug screening, phenotyping and more!

Indirect Calorimetry

OxyletPro uses a standard rodent home cage and uses an airtight lid to ensure the integrity of the sample environment. Simply change from a mouse lid to a rat lid - that is how easy it is to adapt the system for both species.

The home cages are autoclavable, making cleaning easy.

The air supply and switching unit allows for independent flow control to each connected cage and sends the cage samples in tandem to the gas analyzer for O2 and CO2 concentration analysis. Since the flow is independently controlled for each cage, the system has the flexibility to conduct simultaneous experiments of subjects of varying species and/or size, making OxyletPro extremely efficient.

Our gas analyzer features a high quality laser diode O2 sensor and Infrared spectroscopy CO2 sensor, allowing 0.01% resolution.

We offer special configurations for neonatal rats and our single lane airtight treadmills.

Food and Drink Intake

Opt for our airtight lids with transducers to add on food and drink intake monitoring! High precision extensiometric weight transducers are integrated into our airtight lid design and feature easy to access food and drink dispensers.

This extremely stable technology allows intake monitoring with the highest possible accuracy (0.02 g for food and 0.01 g for liquid).

Activity and Rearing

Add on the sensor platform, which houses a third extensiometric weight transducer and continuously record spontaneous activity to clearly identify circadian patterns and activity levels. This highly precise capability will allow you to detect activity without displacement; even the finest movements by mice are detected.

For additional activity monitoring, our IR sensor bars are added to detect occurrence and duration of rearing events.

Modular software package for a modular system

Metabolism offers software modules for respiratory metabolism (METAOXY), intake (METAINT), and activity (METACT) to complement our OxyletPro modular hardware. The NEW user interface features an Experiment Assistant which simplifies and expedites the setup and an Advanced Scheduler Tool for organizing and managing OxyletPro experiments. The Data analysis enhancements include runtime viewers and charts for real time monitoring, batch analysis and an option for data averaging.

Now with an improved, 1-minute switching time, OxyletPro and Metabolism provides greater resolution for the following parameters for each user-defined time interval:

  • VO2/VCO2 concentration
  • VO2/VCO2 consumption
  • Air flow rate
  • Respiratory Quotient (VO2/VCO2)
  • Energy expenditure
  • Treadmill data (if applicable)
  • Food and drink consumption
  • Mean spontaneous activity
  • Rearing data

 

NOTE: the calibration tanks are not provided with the system and should be purchased separately by the user (see specifications below).

Especs.

LE405 Oxygen Sensor

Technology

Laser Diode Absorption

Measurement Range

2-100% (only limited by the high calibration point used)

Resolution

0.01%

Linearity

+/- 0.2%

Noise

+/- 0.03% (20 ms average)

Accuracy

+/- 0.2% (24 hours)

 

LE405 Carbon Dioxide Sensor

Technology

Infrared Spectroscopy

Measurement range

0-10%

Resolution

0.01%

Accuracy

< 10% of reading between 5%-10% CO2; < 0.3% absolute for reading < 5% CO2

 

LE405 Gas Analyzer

L405 Dimensions

260 x 330 x 120 mm (W x D x H)

LE405 Digital output

RS232 (RS232 to USB adapter supplied)

LE405 Analogic Signal Outputs

Connectors: Panel BNC female; Output O2: 10 mV/%O2; Range O2: 0-1V; Output CO2: 100 mV+(100 mV/%CO2); Range CO2: 0.1-1 V

Gas calibration Tanks and regulators needed (not provided by Panlab)

Low point calibration (O2-20%, N2-80%) + High point (O2-50%, CO2-1,5%, N2-48,5%), 10 litres vol., calibration certificate needed with 2 decimals precision, 150 bar pressure, stem output diam. 6 mm inner, 2 identiques pressure regulators 3 bar each.

 

Air flow and switching unit

Standard unit LE400FL Air Flow

0.2 to 2.5 l/min

NeonateUnit LE400FLN Air Flow

0 to 2 l/min

LE400FL Switching

Cycle between 2-4 Chambers in interval running from 1 to 99 min.

Flow Adjustment

Independent regulator for each cage

 

LE1335 platform

Intake Amplifier Resolution

20 mg (for food and drink)

Intake Amplifier Drift

< 0.1 mg/day

IR FRame

11 IR beams spaced 15 mm

 

Home cage

Experimental Chamber Dimension - Rat/mouse

259 (D) x 234 (W) x 209 (H) mm

Experimental Chamber DimensionRat pup

215 and 550 ml

Drink dispenser capacity

Mouse: 44 ml, Rat: 55ml

Food Dispenser capacity

Mouse: 66g, Rat: 75g

 

Room specifications for indirect calorimetry

Room temperature

20-28 °C, the important is that the temperature is maintained stable throughout the experiment.

Ventilation

10-20 room air exchanges per hour

Humidity

30-70 %Rh, the important is that the humidity is maintained stable throughout the experiment.

Barometric pressure

The important is that the barometric pressure is maintained stable throughout the experiment.

Room access

Dedicated closed space with controlled and reduced access to the staff

Publicaciones

SISTEMA OXYLET

 

  • Borck PC et al. (2018) Taurine supplementation induces long-term beneficial effects on glucose homeostasis in ob/ob mice. Amino Acids. 2018 Jun;50(6):765-774. doi: 10.1007/s00726-018-2553-3. Epub 2018 Mar 19. (Physiocage, mouse, Brazil)
  • Singh SP et sl. (2018) CYP-450 Epoxygenase Derived Epoxyeicosatrienoic Acid Contribute To Reversal of Heart Failure in Obesity-Induced Diabetic Cardiomyopathy via PGC-1 α Activation. Cardiovasc Pharm Open Access. 2018;7(1). pii: 233. doi: 10.4172/2329-6607.1000233. Epub 2018 Feb 8. (Physiocage, mouse, USA, China, Israel).
  • Patel V et al. (2018) Coagonist of GLP-1 and glucagon decreases liver inflammation and atherosclerosis in dyslipidemic condition. Chem Biol Interact. 2018 Feb 25;282:13-21. doi: 10.1016/j.cbi.2018.01.004. Epub 2018 Jan 8. (Physiocage, hamster, India)
  • Patel V et al. (2018) Central administration of coagonist of GLP-1 and glucagon receptors improves dyslipidemia. Biomed Pharmacother. 2018 Feb;98:364-371. doi: 10.1016/j.biopha.2017.12.068. Epub 2017 Dec 27. (Physiocage, hamster, India)
  • Zou S et al. (2018) Fatty acid oxidation alleviates the energy deficiency caused by the loss of MPC1 in MPC1+/- mice. Biochem Biophys Res Commun. 2018 Jan 1;495(1):1008-1013. doi: 10.1016/j.bbrc.2017.11.134. Epub 2017 Nov 21. (Physiocage, mouse, China)
  • Dias-Rocha CP et al. (2018) Maternal high-fat diet induces sex-specific endocannabinoid system changes in newborn rats and programs adiposity, energy expenditure and food preference in adulthood. J Nutr Biochem. 2018 Jan;51:56-68. doi: 10.1016/j.jnutbio.2017.09.019. Epub 2017 Oct 5. (Physiocage, rat, Brazil)
  • Lima NDS et al. (2018) Guarana (Paullinia cupana) Stimulates Mitochondrial Biogenesis in Mice Fed High-Fat Diet. Nutrients. 2018 Jan 31;10(2). pii: E165. doi: 10.3390/nu10020165. (Physiocage, mouse, Brazil) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852741/
  • Ogawa-Wong AN et al. (2018) Sexual Dimorphism in the Selenocysteine Lyase Knockout Mouse. Nutrients. 2018 Jan 31;10(2). pii: E159. doi: 10.3390/nu10020159. (Physiocage, mouse, USA)
  • Caimari A et al. (2017) Heat-killed Bifidobacterium animalis subsp. Lactis CECT 8145 increases lean mass and ameliorates metabolic syndrome in cafeteria-fed obese rats. Journal of Functional Foods Volume 38, Part A, November 2017, Pages 251-263 (Physiocage, rat, Spain)
  • Caimari A et al. (2017) Maternal intake of grape seed procyanidins during lactation induces insulin resistance and an adiponectin resistance-like phenotype in rat offspring. Sci Rep. 2017 Oct 3;7(1):12573. doi: 10.1038/s41598-017-12597-9. (Physiocage, rat offspring, Spain)

Contact us for a more complete list of publications.

 

Como pedirlo

HOME CAGE

LE1331

76-0800

760800

LE1331 OxyletPro Home Cage, Requires Lid

 

AIRTIGHT LID OPTIONS

LE1332

76-0801

760801

LE1332 Oxylet Pro Airtight Lid, Rat

LE1333

76-0802

760802

LE1333 Oxylet Pro Airtight Lid, Mouse

LE1338

76-0804

760804

LE1338 Oxylet Pro Airtight Lid, No Intake Monitoring, Rat

LE1339

76-0805

760805

LE1339 Oxylet Pro Airtight Lid, No Intake Monitoring, Mouse

 

HOME CAGE FLOOR OPTIONS

LE1317

76-0806

760806

LE1317 Grid for Rat

LE1318

76-0807

760807

LE1318 Grid for Mouse

LE1312

76-0808

760808

LE1312 Plastic floor with holes for Rat

LE1316

76-0809

760809

LE1316 Plastic floor with holes for Mouse

 

INDIRECT CALORIMETRY HARDWARE

LE405

76-0810

760810

LE405 O2/CO2 Analyzer

LE4002FL

76-0811

760811

LE4002FL  Air Supply and Switching Unit, up to 2 chambers

LE4004FL

76-0812

760812

LE4004FL  Air Supply and Switching Unit, up to 4 chambers

LE1334

76-0823

760823

LE1334 Reference Chamber (one per every 8 cages)

 

INTAKE DISPENSERS

LE1336R

76-0821

760821

LE1336R Drink Dispenser with Bottle, Rat

LE1336M

76-0814

760814

LE1336M Drink Dispenser with Bottle, Mouse

LE1337R

76-0822

760822

LE1337R Food Dispenser, Rat

LE1337M

76-0815

760815

LE1337M Food Dispenser, Mouse

 

INTAKE &ACTIVITY HARDWARE

LE1335

76-0813

760813

LE1335 Intake & Activity Platform

LE1335IP

76-0830

760830

LE1335 Isolation Platform

LE1308

76-0816

760816

LE1308 Rearing Monitoring IR Frames

 

SOFTWARE

METABOLISM V3.0

76-0817

760817

METABOLISM V3.0 Metabolism V3.0 Software, requires software modules

METAOXY

76-0818

760818

METAOXY Indirect Calorimetry Software Module

METAINT

76-0819

760819

METAINT Intake Software Module

METACT

76-0820

760820

METACT Activity Software Module

 

CONSUMABLES & SPARE PARTS

007479

Air filter 0.3 micron

004616

Air filter 0.45 micron

007557

Air filter D65 for LE1334 lid

004653

Nafion tube

 

 

 

 

Cómo pedir una oferta

Contacta nuestro distribuidor el más cercano o contáctanos directamente para recibir una oferta.

Puntos clave

  • Home cage advantage, minimizing stress to the subjects
  • Easily expanded and upgraded as needs grow and change
  • Optimized performance with minimal calibration and maintenance
  • Unmatched versatility with small footprint; adaptable system for mice and rat models
  • Special configurations for neonate testing and treadmill experiments
  • Highly accurate monitoring of food and drink consumption; combining our specially designed dispensers with our weight transducer technology

 

Tests relacionados

Calorimetria indirecta

Ingesta de Comida y bebida

Actividad locomotora circadiana

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