FOUR PLATE box - ARON test

For screening anxiolytic substances in mice

Key features
How to order


The Aron test or four plates test is an animal model of anxiety in which the exploration of the novel surroundings is suppressed by the delivery of a mild electric foot shock.

Basically, the apparatus consists of a cage floored by four identical rectangular metal plates (8×11 cm) separated from one another by a gap of 4 mm. The plates are connected to a shocker unit that can generate electric footshocks.

Following habituation period, the animal is subjected to an electric shock when crossing (transition) from one plate to another, i.e. two legs on one plate and two legs on another. The number of punished crossings is generally calculated for a period of 60 s. A substance with anxiolytic properties induces an increase in the number of punished passages.



Cage materials white, transparent plastic and stainless steel
Dimensions 18 x 25 x 16 cm
Shock 0-3 mA, timer 0-10 sec, square pulse
Shock delivery footswitch
Count manual


  • Andreasen JT et al. (2013) A combined α7 nicotinic acetylcholine receptor agonist and monoamine reuptake inhibitor, NS9775, represents a novel profile with potential benefits in emotional and cognitive disturbances. Neuropharmacology, Volume 73, October 2013, Pages 183–191. (mouse, Denmark, Sweden)
  • Chlon-Rzepa G et al. (2013) New 8-aminoalkyl derivatives of purine-2,6-dione with arylalkyl, allyl or propynyl substituents in position 7, their 5-HT1A, 5-HT2A, and 5-HT7 receptor affinity and pharmacological evaluation. Pharmacological Reports 65: 1529. (mouse, Poland)
  • Hache G et al. (2012) Antinociceptive effects of fluoxetine in a mouse modelof anxiety/depression. NeuroReport 2012, 23:525–529. (mouse, France)
  • Bourin M et al. (2010) Implication of 5-HT2 receptor subtypes in the mechanism of action of the GABAergic compound etifoxine in the ”four-plate” test in Swiss mice. Behavioural Brain Res. 208(2):352-358. (Mouse, France)
  • Foreman MM et al. (2009) Anxiolytic effects of lamotrigine and JZP-4 in the elevated plus maze and in the four plate conflict test. Eur J Pharmacol. 602(2-3):316-20. (mouse, USA)
  • Jacobsen JP et al. (2008) SK3 K+ channel-deficient mice have enhanced dopamine and serotonin release and altered emotional behaviors. Genes Brain Behav. 7(8):836-48. (mouse, Denmark)
  • Masse F et al. (2008) Anxiolytic-like effects of DOI microinjections into the hippocampus (but not the amygdala nor the PAG) in the mice four plates test. Behav. Brain Res. 188(2):291-297 (mouse, France)
  • Mirza NR et al (2008) NS11394 [3'-[5-(1-hydroxy-1-methyl-ethyl)-benzoimidazol-1-yl]-biphenyl-2-carbonitrile], a unique subtype-selective GABAA receptor positive allosteric modulator: in vitro actions, pharmacokinetic properties and in vivo anxiolytic efficacy. J Pharmacol Exp Ther. 327(3):954-68. (mouse, Denmark)
  • Massé F et al. (2007) Anxiolytic-like effect of 5-HT2 ligands and benzodiazepines co-administration: Comparison of two animal models of anxiety (the four-plate test and the elevated plus maze). Behav. Brain Res. 177(2):214-226 (mouse, France)
  • Petit-Demouliere B and Bourin M (2007) Temporal parameters of one-trial tolerance to benzodiazepines in four-plate test-retest. Behav. Brain Res. 183(2):222-225. (mouse, France)
  • Petit-Demouliere B et al. (2007) Factors triggering abolishment of benzodiazepines effects in the four-plate test-retest in mouse. Eur. Neuropsychopharmacol. In press (mouse, France)
  • Massé F et al. (2007) Effect of GABAergic ligands on the anxiolytic-like activity of DOI (a 5-HT(2A/2C) agonist) in the four-plate test in mouse. Eur. Neuropsychopharmacol.;17(6-7):483-91 (mouse, France)
  • Massé F et al (2006) Effect of noradrenergic system on the anxiolytic-like effect of DOI (5-HT2A/2C agonists) in the four-plate test Psychopharmacol. 183(4):471-481. (mouse, France)
  • Ripoll et al. (2006) The four-plates test: anxiolytic or analgesic paradigm? Prog. Neuro-Phychopharm. Biol. Psychiatry. 30(5):873-880. (mouse, France)
  • Dhonnchadha BAN et al. (2005) Implication of 5HT2 receptor subtypes in the mechanism of action of antidepressants in the four plates test. Psychopharmacol. 179(2): 418-429. (mouse, France).
  • Ripoll N et al. (2005) The four-plate test-retest paradigm to discriminate anxiolytic effects. Psychopharmacol. 180(1): 73-83. (mouse, France)

How to order

Four Plate box & Accessory 



Aron Test Box



Shock generator with scrambler. 0 - 2 mA output

How to order

Please contact our local delegates or contact us directly for receiving a quote.

  • Spain: link
  • Other countries: link
  • Key features

    • An elegant and economical solution for screening anxiolytic drugs in mice
    • Punishment based conflict test
    • Shock with adjustable intensity


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