The PANLAB Coupler Module ISO510A is an Amplifier for bridge-system designed to work with a wide range of different transducers for evaluating pressure, strength or respiration in various research areas. As an example, the ISO510 model is currently associated with the isometric or isotonic transducers for the study of in vitro tissues preparations using Organ Baths.

The PANLAB ISO510A Amplifier can work in both AC and DC and amplifies signal up to 2.000 times. The Amplifier contains a filter allowing the suppression of the noise produced by electrical or mechanical artefacts associated with the experiment running (for instance, ventilation and heating pump systems in Organ Baths) and then eliminates vibrations that may affect the signal received from the biological preparations. A Transducer Balance option allows a fine adjustment of the signal baseline (“zero”) when the transducer is not excited by the variable to be measured. The ISO510A amplifier also allows the external monitoring of its output signal, for instance to an oscilloscope…

The ISO510A Amplifier is available in two versions: individual Amplifiers accompanied with the corresponding external Power Supply Box or individual Amplifiers integrated into the Power Supply Box.

Key features

• High Gain Differential AC/DC Amplifier
• Includes a filter allowing the suppression of background noise
• A Transducer Balance allows a fine adjustment of the signal baseline
• Allows external monitoring of its output signal

Specifications

How to order 

Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

Related products

• Transducers (P) – link
• PROTOWIN software (P) - link
• Organ bath for teaching (P) - link
• Organ bath for basic research (P) - link
• Organ bath for advanced research (HS)

Panlab provides complete hardware solutions and associated software for isolated tissues studies from simple, manual systems to automated, computer-controlled systems. 

The mechanical activity of the tissues (muscles) is formed by a displacement (elongation) and a force (contraction). The ratio between elongation and force depends on the properties of the tissue under study: some tissues exhibit a mechanical response with a very large displacement and a reduced force; other tissues produce short but very intense contractions (in terms of strength). Depending on this ratio, the transducer used for performing an organ bath experiment will be isometric or isotonic.

- Isometric transducers

The Panlab Force/Isometric Transducers measures the force of contraction of isolated tissues under conditions of constant force. 

Isometric force transducers are very sensitive to the force applied under conditions contraction with very short amplitude (low displacement). As an example, the Transducers of weight of the electronic scales acts in an isometric way. Generally the isometric technology is also used to measure frequencies; for this reason, the displacement of the sensor of the transducer need to be as slight as possible. Panlab has transducers with a minimum displacement of 0.1 mm for a force application until 25 grams.

Isometric Transducers are classically used in Organ Bath experiments with muscles with short/rapid contractions such as tracheal muscle, atrium, papillary muscle, aortic rings, veins or other vessel rings.

The Transducers are equipped with a special system allowing an easy and rapid fixation of the thread that tightens upper side of the tissue in an organ bath. Panlab provides transducer for various load range, from teaching to standard research systems.

- Isotonic transducers

The Panlab Isotonic Transducer measures displacements with pre-adjusted loads (position transducer).

Isotonic transducers are classically used for measuring the contraction amplitude of isolated muscles in Organ Bath studies, i.e. the changes in tissue length observed under a constant load.

To achieve this capacity, an isotonic transducer must possess a sensor axis which rotates at least 25 degrees. This type of register was the one which was used initially, at the time of the kymographs in such a way that the transducer was configured by a lever, balancing on a pivot.

These transducers are preferably used with smooth muscle preparations characterized by relatively slow contractions (ileum, vas deferens, lung strips, esophagus, urinary bladder, etc.).

Download here the Transducer leaflet – link

Which transducer? The Panlab advice.

Although in principle and from the physiological point of view, the most appropriate for smooth musculature is the isotonic and for heart and striate the isometric, in purity all animal muscle contracts partly isotonically and partly isometrically. Nowadays all the tasks are done with isometric as the response is much more repetitive, the basal line is much more constant, and the relaxation much more rapid. Isotonic transducers are only used in few specific cases

Recommended bibliography:

Kitchen I. Textbook of in vitro practical pharmacology. Blackwell Scientific Publications. Oxford, 1984

Panlab transducer specifications

How to order

Five pin male connectors for Panlab previous ISO510 amplifier are obsolete but can be provided under specific request.   

Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

Related products

• Amplifiers (P) – link
• PROTOWIN software (P) - link
• Organ bath for teaching (P) - link
• Organ bath for basic research (P) - link
• Organ bath for advanced research (HS)

Panlab provides complete hardware solutions and associated software for isolated tissues studies from simple, manual systems to automated, computer-controlled systems. 

The Panlab Compact Organ Baths have been designed and developed to satisfy the requirements of investigators by offering advanced characteristics that render them suitable for the in-vitro study of tissue behavior. Due to their compact design minimum bench space is required.

The modular design allows up to eight individually mounted tissues to be studied simultaneously and independently per system. Several models are available, from one up to eight chambers. All of them are supplied with an external heating control unit, transducer' stands & micropositioners and tissue holders. The main Perspex reservoir houses the heater system, the chambers and coils, all submerged in thermostatically controlled water. Each vessel is filled and emptied by means of a three-way tap (Manual Series) or by electrically-operated valves (Automatic Series) which, besides the possibility of manual activation by means of frontal pushbuttons, can also be remotely controlled by a Timer or Programmer (Software).

All organ baths include tissue holders, transducer holders, micropositioners. The tissue chambers are sold separately.

Panlab can too supply suitable Isometric or Isotonic Force Transducers.

Standard solution for teaching uses the LE120-1 or LE120-2 flat recorders for recording the signals on paper. Optionally, the PANLAB Modular Organ Baths can be associated with transducers, amplifiers and data output software from several brands. 

Cost-saving and straight-to-the-point solution is available as a combination with the Panlab transducers, amplifiers and PROTOWIN software. 

The Panlab organ baths are also the main piece of the ADInstruments organ baths working with the Powerlab and LABChart software systems and proving advanced data report options.

Download here the Panlab Organ bath leaflet – link

Key features

• Compact design thus reducing space requirements to the minimum
• 5, 10, 25 and 50 ml chambers available
• Complete accessibility to all components to facilitate maintenance
• Independent nutrient liquid inputs allowing using different physiological solutions in one same chamber
• All the input-output connections are accessible on the rear panel of the device
• Additional gas outlet (bubbling) to supply oxygen to the preparation into the Petri Capsules
• Two modes for solution replacement: overflow / emptying and filling
• Turbulence-free water circulation
• Use of safety sensors for both water level and water temperature (cut-off at 50 ºC)
• Avoid of thermal inertia using an electronic heating resistance operation control system with incremental gradients
• Available accessories for using continuous perfusion
• Ensures optimal heat stability
• Use of totally inert materials, PTFE, Delrin, Silicone and other materials that are not degraded by acids or salts

How to order

Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath webpage – link

Related products

• Transducers (P) –link
• Amplifiers (P) – link
• PROTOWIN Software (P) - link
• Organ bath for Teaching (P) - link
• Organ bath for advanced research (H)

Panlab provides complete hardware solutions and associated software for isolated tissues studies from simple, manual systems to automated, computer-controlled systems. 

The Panlab Modular Single Chamber Organ Bath was developed to provide a practical and functional design. They are used basically in teaching, where their low demanding maintenance, ruggedness and ease of use guarantee excellent didactic performance. 

This Modular option consists of a Perspex organ bath with a tissue chamber, three-way stopcock for physiological solution exchange and a fine needle valve for gas regulation. 

To maintain a constant temperature in the tissue chamber a Thermoregulated Water Pump is available which can be used with up to four Single Chamber Organ Baths at once. 

All organ baths include tissue holders, transducer holders, micropositiones and tissue chambers (25 ml volume is supplied standard; 5, 10, or 50 ml volumes are available on request). PANLAB S.L. can too supply suitable Isometric or Isotonic Force Transducers.

Standard solution for teaching uses the LE120-1 or LE120-2 flat recorders for recording the signals on paper. Optionally, the PANLAB Modular Organ Baths can be associated with transducers, amplifiers and data output software from several brands. Common configurations use ADInstrument LABCHART or PANLAB PROTOWIN software for data storage and analysis, including Dose/Response.

Download here the Panlab Organ bath leaflet – link

Key features

• Optimal modularity
• 5, 10, 25 and 50 ml chambers available
• Chambers fully and easily interchangeable
• Micropositioner for high precission adjustment of the Transducer and tissue-holder support
• Complete accessibility to all components to facilitate maintenance or replacement
• Extremely easy to place the stimulation electrodes
• Independent valves per chamber for the fine adjustment of bubbling
• Minimum drift of the nutrient liquid
• Use of totally inert materials, PTFE, Delrin, Silicone and other materials that are not degraded by acids or salts

How to order

Isolated organ/issue test & Integrated solutions

For more details about the isolated organ/tissue method, consult the following link

For more details about standard integrated organ bath solutions, consult the “Related Integrated solutions” section of the Isolated organ/tissue test – organ bath• webpage – link

Related products

• Transducers (P) –link
• Amplifiers (P) – link
• PROTOWIN Software (P) - link
• Organ bath for basic research (P) - link
• Organ bath for advanced research (H)

El método de medición de la presión arterial incruenta de manera no invasiva (NIBP) tiene como objetivo evaluar la presión arterial sistémica y los parámetros cardiovasculares en roedores sin el uso cateterismo invasivo. 

Panlab NIPB system provides an easy and reliable technique to measure systemic blood pressure and cardiovascular parameters in rodents without any invasive catheterization.

The system is composed of a NIBP control unit, pressure cuff & pulse transducer, a heater, restrainers and optional software options. Different models of these components are available depending on the experiment request.

The pulse signal level (heart rate) is permanently monitored on the LCD display of the NIBP control unit while an internal pressure transducer is constantly measuring the current pressure applied on the cuff. Measurement process involves several steps which are performed automatically; systolic and diastolic blood pressure and calculated mean blood pressure are visualized in real-time in the digital display of the control unit. With regards to the systolic pressure, it can be achieved either once the heart pulse disappears or else when the first pulse reappears (user selectable) after the occlusion process.

For more details about the NIBP method, consult the Non Invasive Blood Pressure test link

All NIBP units can be indistinctly used for rats, mice or dogs, using specific pulse transducers & cuff. A built-in air pump ensures automated cuff inflation/deflation at constant and even rate. In order to avoid deterioration of the animal’s tail after submitting animals to unnecessary high occlusion pressures, the automated deflation is carried out for each animal and separately, once their own systolic value is reached. Real values (without linear regression calculation) are thus achieved while remaining easily traceable when using the NIBPChart option. 

The Automatic Heater & Scanner option allows a sequential measurement of 6 to 12 animals without having to change pulse transducers and tail cuffs.

The requirement of a warm environment for the animals is critical for accurate blood pressure measurements. To provide adequate conditions for NIBP measurement, the system can be used in association to a warming chamber which provides a comfortable and heated environment necessary to produce peripheral vasodilatation and isolate the animal from external noise.

Download here the Panlab NIBP leaflet – link 

Data output options

NIBP CONTROL UNITS:

The NIBP data can be seen directly on the display of these control units: Systolic blood pressure, Diastolic blood pressure, Mean blood pressure and Heart rate. 

SEDACOM V2.0 + CONRS232USBHS

Software option allowing the user transferring to the SEDACOM software the same data shown on the NIBP control unit display. These data will be shown in Numeric table directly exportable to Excel. Together with these numeric data, SEDACOM will provide information about the session (date, etc…) and allows the user entering information about the subjects under experiment. For more information about the SEDACOM software, go to the next link. 

NIBPCHART + NIBPCHARTUSB

Data output in this case is graphical! The NIBPchart software is an option system offering a straightforward way to ensure the traceability and validation of the data provided by the NIBP units. The evolution of animal pulse and cuff pressure signals are visualized in real time in the screen and the registration can be stored by the user for the further interpretation of the measurement. The values of the systolic and diastolic blood pressures can be determined graphically by browsing the registered signals with the cursor and a specific data panel automatically displays the values of the cuff pressure related to the cursor displacements. Marks indicating the value of the systolic and diastolic pressures can be left on the signal by the user as memorandum of the graphical determination. Once the pressure marks are established, the graphical representation of the measurement can be exported to an image file in BMP format. This is a very important data output for ensuring that the measurement is done in reliable way, so we always strongly recommend the user to have it. The NIBP test success mainly depends on the animal habituation so, this software is the best way to see whether the animal is ready or not for the measurement. This is also the best way to check the reliability of the data.

More detail about the NIBPchart features in the following link. 

Open-field experiment allows the evaluation of animal’s basal activity and its evolution along the time, in response to novelty or anxiogenic environment, and in response to pharmacological treatment, lesion or genetic modification.

The 5-choice serial reaction time (5CSRT) task is commonly used to evaluate attention performance using visual discrimination in laboratory animals. It represents a conditioning paradigm, involving intact attention processes.

The weight bearing test represents an unsurpassed method for assessing spontaneous pain and postural deficits in laboratory animals.

La prueba de la retirada de la cola (Tail-Flick Test o prueba de D’Armour & Smith) es una prueba que evalua la sensibilidad al dolor mediante de la evaluación de la latencia de retirada de la cola en respuesta a un estímulo térmico en rata y ratón.