The apomorphine test using an rotameter has proved to be a popular test for screening the behavioral effects of a wide variety of lesions, drugs, and other experimental manipulations on the brain of rodents.
Forced exercise using a treadmill in rodents is a useful tool with a great value in the study of functional capacity and is a validated standard model for investigations in the field of human metabolism.
The novel object test is a free exploration paradigm that provides animals the opportunity to explore a novel object in a familiar environmental context.
The emergence test is a free exploration paradigm designed to reduce anxiety by providing a safe enclosure within the open field in order to assess approach or exploratory behavior in rodents.
Locomotor responses to novelty is an animal index of exploration/anxiety that has been shown to represent a predictive factor for the addictive properties of drug or animal vulnerable to drug.
The rodent Activity Wheel represents a very simple and clever way to register animal physical activity in its home cage environment.
The holeboard test is mainly used for assessing exploratory behaviors in rodents.
Open-field experiment allows the evaluation of animal’s basal activity and its evolution along the time, in response to novelty or anxiogenic environment, and in response to pharmacological treatment, lesion or genetic modification.
VivaMARS is a new activity solution specially designed to address all the needs of high efficiency in CROs for neurotoxicology and neuropharmacology studies.
Features & Benefits
Partnership with Data Science International (DSI)
DSI and Panlab brands, divisions of Harvard Bioscience Inc., are leading manufacturer of quality instrumentation for animal behavioral and physiology research. Representing over 50 years of experience and referenced in more than 7,000 peer-reviewed publications, our solutions are proven choices for a wide range of in vivo applications spanning neuroscience, toxicology, pharmacology and more. Our collective team is eager to share our expertise and support your research as we have with many of the world’s scientists for decades.
In the context of this partnership, DSI and Panlab features the new VivaMARS solution, a high-capacity activity monitoring system for pre-clinical applications.
The new system combines the Company’s high precision Panlab activity monitoring technology with the industry leading Ponemah™ pre-clinical data management software to create an integrated good laboratory practice (GLP) compliant solution. The system is ideally suited to meet the high throughput, automated pre-clinical safety and pharmacology testing needs of CROs and pharma, in addition to acute activity studies carried out by leading research and academic institutes.
For more information visit VivaMARS website
Smart I/O is an enhanced version of the Panlab SMART 3.0 video-tracking for the combined evaluation of behavior with the neural recording and stimulation (electrical and optogenetics).
The importance of the use of video recording and video tracking tools is signifi cant in this context for ensuring a reliable assessment of behavior in neuroscience research.
Panlab features the new OxyletPro waste collection cage for mice. A more comprehensive approach to understanding the animal body mass phenotype by combining the assessment of respiratory metabolism with digestive efficiency through the analysis of collected urine and feces.
OxyletPro’s full modular design allows for simple expandability. Configurations are available for home cage metabolism phenotyping with our Physiocage system and for cardiovascular studies with exercise physiology with our airtight treadmills.
Start with the configuration that meets your requirements today and expand as needs change and grow!
OxyletPro can be used in the following applications: obesity, diabetes, metabolic disorders, nutrition studies, drug screening, phenotyping and more!
RECORD-IT! MEDIA is the Panlab product specifically designed to record digital videos in laboratories and scientific environments in general.
RECORD-IT! MEDIA can record the live images of up to 8 cameras simultaneously into independent digital video files. RECORD-IT! MEDIA also provides a Player for the review of the recorder videos. Both video recording and video playing can be synchronized with third-party system.
RECORD-IT! MEDIA processes the images (frames) coming from the connected images sources (cameras). The cameras supported by RECORD-IT! MEDIA are digital USB cameras, webcam, WIA cameras, analog cameras and IP network cameras. RECORD-IT! MEDIA also provides some simulated live image video sources for demo purposes.
RECORD-IT! MEDIA player is compatible with a wide range of video formats.
OxyletPro is a modular system integrating:
OxyletPro, an optimized system for studies in laboratory research models, utilizes indirect calorimetry to evaluate respiratory metabolism.
Food and drink intake and activity are evaluated using Panlab’s weight transducer technology. This highly stable technology permits the continuous assessment of consumption and spontaneous activity with superior accuracy. For a more comprehensive evaluation of activity, an Infrared (IR) frame can be added to monitor rearing behavior.
OxyletPro’s unique modular design allows for simple expandability. Start with the configuration that meets your requirements today, and expands as needs change and grow.
Special configurations are available for calorimetry studies with neonatal rat pups as well as exercise physiology studies with our single lane, airtight treadmills.
OxyletPro can be used in the following applications:
Obesity, diabetes, metabolic disorders, nutrition studies, chronobiology/circadian rhythm studies, drug screening, phenotyping and more!
OxyletPro uses a standard rodent home cage and uses an airtight lid to ensure the integrity of the sample environment. Simply change from a mouse lid to a rat lid - that is how easy it is to adapt the system for both species.
The home cages are autoclavable, making cleaning easy.
The air supply and switching unit allows for independent flow control to each connected cage and sends the cage samples in tandem to the gas analyzer for O2 and CO2 concentration analysis. Since the flow is independently controlled for each cage, the system has the flexibility to conduct simultaneous experiments of subjects of varying species and/or size, making OxyletPro extremely efficient.
Our gas analyzer features a high quality laser diode O2 sensor and Infrared spectroscopy CO2 sensor, allowing 0.01% resolution.
We offer special configurations for neonatal rats and our single lane airtight treadmills.
Opt for our airtight lids with transducers to add on food and drink intake monitoring! High precision extensiometric weight transducers are integrated into our airtight lid design and feature easy to access food and drink dispensers.
This extremely stable technology allows intake monitoring with the highest possible accuracy (0.02 g for food and 0.01 g for liquid).
Add on the sensor platform, which houses a third extensiometric weight transducer and continuously record spontaneous activity to clearly identify circadian patterns and activity levels. This highly precise capability will allow you to detect activity without displacement; even the finest movements by mice are detected.
For additional activity monitoring, our IR sensor bars are added to detect occurrence and duration of rearing events.
Metabolism offers software modules for respiratory metabolism (METAOXY), intake (METAINT), and activity (METACT) to complement our OxyletPro modular hardware. The NEW user interface features an Experiment Assistant which simplifies and expedites the setup and an Advanced Scheduler Tool for organizing and managing OxyletPro experiments. The Data analysis enhancements include runtime viewers and charts for real time monitoring, batch analysis and an option for data averaging.
Now with an improved, 1-minute switching time, OxyletPro and Metabolism provides greater resolution for the following parameters for each user-defined time interval:
NOTE: the calibration tanks are not provided with the system and should be purchased separately by the user (see specifications below).
The Dynamic Weight Bearing - New Incapacitance Test, is a new test for assessing spontaneous pain in freely moving rodents, which is based on an instrumented-floor cage and a combined video acquisition system. The Dynamic Weight Bearing test, especially suitable for research on Parkinson and allodynia, was a major break-through in the field of research on analgesia: operator-independent, time-saving, convenient for manipulating large amounts of rodents, and induces no stress on the animal (rat or mouse). The system has been used extensively by many customers from private firms to academic labs since 2008 for various types of research on analgesia and nociception.
The Dynamic Weight Bearing system also offers the possibility of conducting the experiment over a longer observational period (5 minutes) without an operator (thus reducing stress on the animal), and without any habituation period. With these exclusive features, the DWB opens a new and improved generation of instruments for drugs screening and optimization of preclinical models. The latest version is now a semi-automatic, operator-independent instrument.
Performing the same measurements as the original manual Version, the Advanced DWB (Dynamic Weight Bearing) improves two of the turnkey points of the system: the speed of operation and the operator independent factor. The Advanced DWB allows the user to reduce the most important phase of the process by a factor of 8 to 10, thus the analysis of 1 animal will take 1 to 2 minutes and be operator-independent.
The results encompass a lot of valuable information but will ask for a minimum of manpower and many more animal analysis can be done in the same amount of time than with a manual instrument. The software gives the weight distribution of the animal (rat or mouse), per limb, with additional ratio computations and filtering options.
Analysis and replay can be performed on site or remotely using Bioseb's exclusive software for Dynamic Weight Bearing and Incapacitance tests. During the analysis and replay, the operator can check and secure each limb recognition for hind and front paws. The weight distribution of the animal (rat or mouse) per limb is then shown in the result window, for each time period with the mean and the variation coefficient.
The Kinetic Weight Bearing (KWB) system is an alternative method generating quantitative data on footprints and gait in spontaneously moving animals.
The unique "sensor-mat" technology originally designed by Bioseb for the Dynamic Weight Bearing application has been refined and adapted to make kinetic analysis possible. This allows the weight borne by each individual paw to be tracked during a walking sequence in a corridor. The Kinetic Weight Bearing Instrument provides additional information on applied weight as well as the speed and acceleration of each paw as the animal moves toward an end point of the runway platform. This additional information provides data on coordination and gait comparison paw to paw and step to step. The system combines the video of a freely walking rodent (mouse or rat) with the pressure applied by each paw in real time using an array of not less than 4000 or 6000 sensors depending on the corridor's length.
The Kinetic Weight Bearing (KWB) Instrument offers automated parameter analysis for each paw of a rodent on Maximum Force (mN), Mass Velocity (N/s), and Maximum surface area (cm2). Additional Gait Parameters are also analyzed, speed, cadence, overlaps, and step patterns, are all compared and statistically processed. An automated analysis package is available for automatic analysis of a batch of experiments, saving time in analysis, and making the results more consistent and less operator dependent.
The instrument combines the video of a freely moving rodent with the data supplied by the mat including up to 6000 sensors that cover the full length of a corridor at the end of which lies the animal's home cage. Gait analysis can be carried out, and can be applied in the study of diseases such as CNS ischemia, SCI, and Neurotrauma, for example.
Bioseb has demonstrated that the rising time to the maximum force of each animal step differs across different animal models. The KWB is able to compare the path of the animal's "Geometric Center of Gravity" with the actual "Weighted Center of Gravity", and show significant differences. Changes in weight distribution from left to right, front to back and contra-lateral compensation are easily measured, thus providing useful information for your research work on nociception and analgesia.
Gait analysis has been extensively used in phenotyping of CNS diseases such as cerebral ischemia, neurodegenerative diseases, Spinal Cord Injury models and some pain models like neurotrauma (Nerve Crush, SNI…). The kinetic weight bearing system adds a major feature to existing gait analysis systems by including the measurement of the force applied by each individual paw to the floor the animal is walking upon.
The OxyletPro system is a modular system allowing the integration of respiratory metabolism (O2 consumption /CO2 production). Panlab provides a complete Oxylet solution enabling forced exercise training in a treadmill combined with indirect calorimetry in rodents.
Basically, Panlab Treadmill apparatus consists of a rolling belt with adjustable speed (up to 150 cm/s) and slope (from -25 to 25 degrees) and a control Unit. The rolling belt is built with especially selected materials to guarantee the best performance under conditions of intensive use and the minimum operations of maintenance, as well as simplicity in keeping it clean. The lanes (corridors of activity for the animal) have sufficient width for the subject to correct its errors in coordination, thereby allowing an exact measurement of the fatigue without deficiencies in motor coordination.
The treadmill unit controls the speed of the belt, shows measured data in its touchscreen display and provides electrical shock to the grid. The electrical shock supplied by the grid is of constant intensity (from 0 to 2 mA), that is, the current which circulates through the animal (and therefore its effect) only depends on the value of the mA chosen and not of the subject (quantity of body mass in contact with the bars, perspiration, etc.)
For metabolism studies, the treadmill is provided with an air isolated enclosure. The LE400 air flow control unit allows a fine regulation of the air flow inside the treadmill and sends the air to the LE450 gas analyzer for O2/CO2 gas concentrations determination.
The associated METABOLISM software transfers the data from the treadmill control unit and the gas analyzer to a PC computer using RS232/USB outputs for data storage and further analysis.
NOTE: the calibration tanks are not provided with the system and should be purchased separately by the user (see Specifications tab).
METABOLISM software is the last element of the chain of components of the Panlab OXYLET,FOOD and DRINK and PHYSIOCAGE systems. METABOLISM allows the extraction of the data obtained from these Panlab devices as well as the calculation of important parameters for physiological studies. A combined evaluation of the respiration metabolism, food/drink intake, spontaneous activity and rearing is then rendered possible by the use of this very simple and easy-to-use software.
METABOLISM consists in different modules, activated upon customer request:
The program gets data in digital form from the Panlab devices. METABOLISM can also import and convert analogical data from the *.txt files generated by Data Acquisition Systems (PowerLab® recommended).
Gathered data can be processed and re-processed using different time intervals of calculation. The program displays a Data Table which can be saved in Excel format for further analyses.
Compulse represents the software component of the Panlab PheCOMP system designed for the assessment of compulsive behavior in rodents.
This software monitors up to 12 PheCOMP cages for data acquisition, storage and analysis. Up to 4 external units for food or drink by cage can be set (user-defined combination and name) ensuring an optimal flexibility for performing a wide variety of tests related to Food & Drink Intake studies.
The evolution of food and drink intake consumption, beams break activity, rearing and current animal position are displayed in real-time during the data acquisition process. The data are stored in two data files: MTB file opened from the Compulse software for Intake & Meal Pattern Analysis and TAC file opened from the Actitrack software for Global Activity, Tracking & Rearing Analysis.
A powerful analysis module provides tables with the calculation of total and accumulated intake for each dispenser in each cage as well as all the standard data for meal pattern analysis. The analysis window and subinterval are defined by the user. Data can also be displayed graphically. All the tables and graphs are easily exportable in Excel o BMP formats, respectively.
The rodent Activity Wheel represents a very simple and clever way to register animal physical activity in its home cage environment.
The use of this high throughput tool is particularly relevant for research involving circadian rhythms, phenotyping and drug testing.
Basically the animals are housed individually in the home cages equipped with the running wheel. The total number of wheel rotation made by the animal is displayed on the external LE907 individual counter or LE3806 multicounter devices.
LE3806 multicounter allows storing the data in userdefined time intervals. The data can be visualized directly from the multicounter display and/or exported to the SeDaCom PC interface (through RS 232 serial port) in a format compatible with Excel. The new SEDACOM 2.0 version (not included with the LE3806 multicounter) provides an easy and convenient way to visualize and export the data (number of total rotations by user-defined interval of time) on a computer for further analysis.
All the components of the wheel assembly (wheel, wheel hub and support) are made of stainless steel and are used with standard ACE (Allentown Caging Equipment) polycarbonate rodent cages provided with its wire lid. The wheel is built outside the home cage to preserve animal life space.
All non-electrical cage components are autoclavable.
ActiTrack controls data coming from up to 32 IR frames. It discriminates and analyses frequency and number of IR beam breaks to convert it in a track file that can be analysed later for generating reports. Thus ActiTrack provides much more integrated and precise data in terms of animal activity, position, displacements and rearings than those provided by the SeDaCom software.
Ideal for exercise training and fatigue studies in rodents, Panlab’s treadmills are recognized as some of the most sophisticated solutions on the market.
Treadmills are a popular tool for studying the physiological adaptations and possible benefits resulting from exercise. Applications span a wide range of research interests including sports performance, metabolic diseases, cardiovascular disorders, spinal cord injury recovery and chronic pain models.
Panlab/Harvard Apparatus small animal treadmills are used for forced exercise training and accurate testing of fatigue in rodents. A control unit with touchscreen user interface plus the ability to perform individual lane stimulation (shock or air-puff) add convenience and experimental flexibility in setting up and managing study parameters.
The treadmill includes a shock grid which delivers electrical shock of constant intensity. An air-puff accessory option (air source not included) is interchangeable with shock, enabling the use of one or the other depending on experimental needs. The treadmill lanes have sufficient width for the animal to correct any errors in coordination, allowing an exact measurement of fatigue. Robust design and high-quality materials guarantee high performance under conditions of intensive use.
The control unit’s touchscreen allows control of the speed of the belt and the intensity of the stimulus. Parameters measured are recorded and shown on the control unit display, including belt speed and slope, distance covered, accumulated shock time, and shock intensity. The control unit provides current to the shocking grid and allows communication with the PC for data storage through the optional SEDACOM software (USB communication). The SEDACOM software is needed for editing and executing user-defined protocols of speed (acceleration, deceleration, customized, etc.). The sessions can be manually stopped independently in each lane or automatically using specific user-defined parameters.
We also propose single lane treadmill configurations for metabolism studies (indirect calorimetry).
A new speed range of 0.4 to 150 cm/s allows further flexibility in exercise and fatigue studies and opens its use to new fields of applications (non-rodent animal species, fine motor coordination…).
The new speed range would be available in all standard touchscreen treadmills except for the rabbit treadmill model.
For the OxyletPro airtight treadmill (Metabolism studies), the new low speed range is not available through the METABOLISM software (5 to 150 cm/s)
Please contact us for getting more information about how to upgrade the firmware of your touchscreen treadmill and get this new low speed options along together with some minor improvements/bug corrections.
Open-field experiments allow the evaluation of animal’s basal activity and its evolution, in response to novelty or anxiogenic environment, and in response to pharmacological treatment, lesion or genetic modification.
Panlab proposes square open-fields available for rats and mice. The arena is made of durable material which has the advantage to be non-absorbent to the odours and easy to clean. The arena is surrounded by high walls and is available in different non reflective colours for video-tracking purposes. The system is entirely demountable for enabling storage in the minimum space. The floor can be divided into equal squares under request for the direct counting of animal activity.
The Panlab open-field can be combined with the SMART video-tracking system for the automated evaluation of a wide variety of behaviors (activity, exploration, anxiety, etc...)
The Panlab Infrared (IR) Actimeter allows the study of spontaneous locomotor activity, rearings and optionally hole-board test parameters for exploration in rodents. A reliable system for easy and rapid drug screening and phenotype characterisation in both day and night lighting conditions.
The system is basically composed by a 2 dimensional (X and Y axes) square frame, a frame support and a control unit. Each frame counts with 16 x 16 infrared beams for optimal subject detection.
The system is completely modular: each frame may be used for evaluation of general activity (one or several animals), locomotor and stereotyped movements or rearings or exploration (nose-spoke detection in the hole-board option). The infrared photocell system can be set with up to 15 levels of sensitivity in order to adapt the frames to the typology of the animal (rats, mice). It can also be set to ignore the beams that are obstructed by objects (e.g. the walls/corners of the home cage).
The frames can be controlled by the independent control units LE8825 or directly through the optional SEDACOM computer software. The new SEDACOM 2.0 version provides an easy and convenient way to visualize and export the data on a computer for further analysis.
Optionally, the ActiTrack software option may be used to analyse animal trajectories (distance, speed, permanence time in selected zones) and then provide additional complementary data to those obtained using the control units.
Panlab circular pool provides the ideal environment to carry out the Morris water maze test (MWM test) and aquatic radial maze tests for the study of memory in rodents.
The water maze tank is manufactured in polypropylene and stands on a support with 4 wheels for easier displacement. Panlab proposes a complete solution for water maze settings since the heater, the water circulation pump, the level controller and the electro valve for pool filling are containing in a unique control box. The level controller acts directly on the Electro Valve, turning it off when the liquid arrives to the corresponding height. The water temperature is thermostated between 22 and 32 degrees depending on the environmental room temperature.
For Morris water maze (MWM) Test, a platform support is provided with two easily interchangeable platforms (80 and 110 mm) that can be located anywhere in the water maze.
For aquatic radial maze, a removable-floating eight radial-arm maze structure and associated platform can be provided upon request.
Both Morris water maze and aquatic radial mazes can be associated with the SMART Video-Tracking software for detection and analysis of animal displacements and behavior throughout the test.
The new SEDACOM 2.0 version is a very easy, convenient and cost-saving data transfer software providing an ideal environment for visualizing the registered data on a computer and exporting them in a format that simplifies any further post-analysis processes. SEDACOM increases the functionalities of the devices, saving and listing automatically all the data of the current or stored sessions.
SEDACOM can be used with a wide range of devices from several lines of Panlab products for measuring physiology and behaviour in small laboratory animals (motor activity, pain sensitivity, body temperature, memory etc.)
The name of SEDACOM comes from SErial DAta COMmunication due to the direct communication of the Panlab devices to the computer through an RS232 serial port communication. Nowadays, some optional accessories are offered for allowing the use of the USB technology making possible running experiment on a laptop, if requested.
SeDaCom can adquire data from up to 9 devices at the same time, limited exclusively by the number of series ports available on the computer. An additional hub or board with serial ports (not included) can be installed to expand the number of connections. SeDaCom will automatically recognize the number of systems connected.
Smart 3.0, la última versión del sistema de video-tracking SMART lo hace todavía más flexible e intuitivo dentro del contexto de su uso para la evaluación automatizada de la conducta en una gran variedad de aplicaciones pre-clínicas y en neurociencias.
El SMART proporciona datos relevantes para resolver problemas básicos de psicofarmacología e de investigación clínica. Es una herramienta que se puede utilizar para tareas de fenotipado (diferencias entre cepas, efecto de una modificación genética, etc.) y para el estudio de los efectos de sustancias farmacológicas sobre el comportamiento en animales de laboratorio.
Utilizando el análisis avanzado de imágenes, SMART 3.0 permite el registro de las trayectorias de los animales, de eventos definidos por el experimentador, de la interacción social entre diferentes individuos y de su actividad global. SMART 3.0 ofrece a sus usuarios toda la flexibilidad de un sistema modular.
SMART 3.0 fue desarrollado de manera a tomar en cuenta las tareas diarias relacionadas con los procedimientos experimentales – una interfaz fácil de usar, una estructura altamente flexible para adaptarse a la mayoría de las aplicaciones y presupuestos. Sus características avanzadas garantizan la obtención de datos fiables y una mayor productividad, ahorrando tiempo y recursos.
SMART 3.0 se caracteriza por su grande flexibilidad, productividad y simplicidad – el experimentador solo tiene que ajustarle a sus experimentos, SMART 3.0 hará el resto.
Simply SMART, simply powerful.